Simple and rapid method for the confirmation of bacterial colonies via PCR using the preparation of a suspension of bacterial colonies or pure cultures. Ideal for colony confirmation via PCR of e.g. Legionella or Salmonella (S. Enteritidis, S. Typhimurium), or for serogroup determination of STEC colonies. Reliable confirmation of Legionella colonies from agar plates according to ISO 11731:2019. Bacteria in the suspension can be recultivated.